Culture condition design
Rotation conditions, culture density, culture period, and medium conditions are adjusted for the target research purpose.
Rotary culture for stem cell function design.
Cell Reborn LLC uses rotary culture instead of T-flask culture and connects culture conditions to secretome quality, anti-proliferative evaluation, and cell activation potential.
Core Technology
A T-flask-free rotary culture process.
Stem cell culture supernatant and conditioned medium vary with cell state, passage history, culture duration, medium composition, and collection timing. Cell Reborn LLC manages these variables through rotary culture and links them to Miyakawa Method-based evaluation.
- Rotary culture without T-flask culture
- Stem cell culture supernatant and conditioned medium preparation
- Functional evaluation using Miyakawa Method and cell activation potential
- Related patents granted in Japan and the United States
Secretome preparation
Collection, preprocessing, storage, and lot-to-lot comparison are designed for research-grade supernatant and conditioned medium.
Function-linked quality
Anti-proliferative activity against cancer cells, cell activation potential, cytotoxicity, secretome, and quality indicators are evaluated together.
Culture Method
Technical features
| Culture format | Rotary culture without T-flask culture |
|---|---|
| Objective | Control the culture environment and connect stem cell culture, supernatant, and conditioned medium to functional evaluation |
| Evaluation axis | Miyakawa Method-based evaluation of anti-proliferative activity against cancer cells, cell activation potential, cytotoxicity, secretome, and quality indicators |
| Intellectual property | Related patents have been granted in Japan and the United States |
Scope
A research platform designed with safety evaluation in mind.
The technology description is for R&D-stage communication. It does not claim clinical efficacy, diagnosis, treatment, prevention, approval, or guaranteed safety.